Uric Acid Test

Uric acid is a heterocyclic compound of carbon, nitrogen, oxygen, and hydrogen with the formula C₅H₄N₄O₃. It forms ions and salts known as urates and acid urates, such as ammonium acid urate. Uric acid is a product of the metabolic breakdown of purine nucleotides, and it is a normal component of urine.

Uricase enzyme in the reagent converts uric acid to Allantion and hydrogen peroxide. the hydrogen peroxide formed further reacts with phennolic compound and 4 aminoantipyrine by caatalytic action of peroxidase to form pink to red color. Intensity of the color is proportional to the concentration of uric acid in the sample.

1. Prepare 3 Tubes and add 1,0000 ul from uric acid reagent in each Tube
2. Label the tubes (Blank, Standard, Test)
3. Leave Blank tube . Add 20ul from sample to the Test Tube, and add 20ul from Standard to the Standard Tube
4. Mix Well and incubate 10 mins @ room tempreature or 5 mins @37c
5. Read the Absorbance against blank at Wavelenght 520 nm