What is Uric Acid
Uric acid is a heterocyclic compound of carbon, nitrogen, oxygen, and hydrogen with the formula C₅H₄N₄O₃. It forms ions and salts known as urates and acid urates, such as ammonium acid urate. Uric acid is a product of the metabolic breakdown of purine nucleotides, and it is a normal component of urine.
Principle
Uricase enzyme in the reagent converts uric acid to Allantion and hydrogen peroxide. the hydrogen peroxide formed further reacts with phennolic compound and 4 aminoantipyrine by caatalytic action of peroxidase to form pink to red color. Intensity of the color is proportional to the concentration of uric acid in the sample.
Sample Type
Serum or Plasma
Procedure
- Prepare 3 Tubes and add 1,0000 ul from uric acid reagent in each Tube
- Label the tubes (Blank, Standard, Test)
- Leave Blank tube . Add 20ul from sample to the Test Tube, and add 20ul from Standard to the Standard Tube
- Mix Well and incubate 10 mins @ room tempreature or 5 mins @37c
- Read the Absorbance against blank at Wavelenght 520 nm
| Blank | Test | Standard | |
| Uric Acid Reagent | 1,000 μl | 1,000 μl | 1,000 μl |
| Sample (Serum/ Plasma | – | 20 μl | – |
| Standard | – | – | 20 μl |
Normal Values
Calculations
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