Skip to content
View Categories

Urease Test

1 min read

INTRODUCTION

The urease test detects the enzyme urease produced by some bacteria, allowing differentiation and identification of certain species. It determines an organism’s ability to split urea into carbon dioxide and ammonia via the urease enzyme. A positive test produces an alkaline pH detected by a pH indicator dye color change. Rapid urease tests can help diagnose certain infections.

Test Catageory
فئة التحليل

Microbiology

Refrence Books
كتب مراجع

1. Bailey & Scott’s Diagnostic Microbiology
2. Laboratory Manual and Workbook in Microbiology

Skills Required
المهارات المطلوبة

1. Inoculation
2. Incubation
3. Color change interpretation

Video Resources
مصادر فيديو

Resource 1
Resourse 2
عربي

Principle

Urease broth contains urea substrate and a pH indicator. Bacteria producing urease enzyme hydrolyze urea into ammonia causing alkalinization and color change of phenol red pH indicator, detecting urease activity.

Solutions & Equipments

1. Urease test broth
2. Bacterial isolates
3. Inoculation loop
4. Test tubes
5. Incubator

Sample Type

Pure bacterial isolates from primary culture requiring identification.

Procedure

1. Heavily inoculate urease broth with test organism
2. Incubate aerobically for up to 24 hours at optimal temperature
3. Check periodically for color change

Results

No color change = negative result (no urease) Pink/red color = positive result (urease produced)

Notes: 

1. Test known urease positive/negative strains
2. Do not excessively incubate negative tests
3. Ensure adequate inoculation

Quick Notice

M. Sulieman

mohammad@mlsgaate.com

Test known urease positive/negative strains
Do not excessively incubate negative tests
Ensure adequate inoculation

Powered by BetterDocs

PHP Code Snippets Powered By : XYZScripts.com