Test Catageory
فئة التحليل
Microbiology
Refrence Books
كتب مراجع
1. Bailey & Scott’s Diagnostic Microbiology
2. Laboratory Manual and Workbook in Microbiology
Skills Required
المهارات المطلوبة
1. Inoculation
2. Incubation
3. Color change interpretation
Video Resources
مصادر فيديو
Principle
Urease broth contains urea substrate and a pH indicator. Bacteria producing urease enzyme hydrolyze urea into ammonia causing alkalinization and color change of phenol red pH indicator, detecting urease activity.
Solutions & Equipments
1. Urease test broth
2. Bacterial isolates
3. Inoculation loop
4. Test tubes
5. Incubator
Sample Type
Pure bacterial isolates from primary culture requiring identification.
Procedure
1. Heavily inoculate urease broth with test organism
2. Incubate aerobically for up to 24 hours at optimal temperature
3. Check periodically for color change
Results
No color change = negative result (no urease) Pink/red color = positive result (urease produced)
Notes:
1. Test known urease positive/negative strains
2. Do not excessively incubate negative tests
3. Ensure adequate inoculation
M. Sulieman
mohammad@mlsgaate.com
Test known urease positive/negative strains
Do not excessively incubate negative tests
Ensure adequate inoculation