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Triglycerides Test

1 min read

INTRODUCTION

Triglycerides are the main type of fat in the body, and they provide a major source of energy. However, high levels of triglycerides can be a risk factor for heart disease, stroke, and pancreatitis.

Test Catageory
فئة التحليل

Clinical Chemistry

Refrence Books
كتب مراجع

1. Bishop’s Clinical chemistry

Skills Required
المهارات المطلوبة

1. Pipetting
2. Colorimetry

01

Video Resources
مصادر فيديو

Resource 1
Resourse 2
عربي

Principle

Triglycerides are measured enzymatically in serum using a series of coupled reactions in which triglycerides are hydrolyzed to produce glycerol. Glycerol is then oxidized using glycerol oxidase, and H2O2, one of the reaction products, is measured as described above for cholesterol. Absorbance is measured at 500 nm.

Equipments

1. 10 – 100 ul + 100 – 100 ul Automatic Pipette
2. Small glss tubes
3. cholesterol mono-reagent
4. yellow and blue tips
5. Colorimeter or Spectrophotometer

Sample Type

Serum or plasma is acceptable for the Test
But make sure the Patient is fasting to get accurate results

Procedure

1. in 3 clean dry tubes add reagents and sample as mentioned in the Table below

Blank (B)STandard (STD)Test (T)
W. Reagent1000 μl1000 μl1000 μl
Standard10 μl
Sample10 μl

Procedure

2. Mix Well and incubate for 10 mins at room Tempreature or 5 min @ 37c’
3. using the colorimeter or spectrophotometer read the absorbance against blank at filter wavelenght 540 nm
4. then do the calculations based on the following formula

Cholesterol = Absorbance of test / Absorbance of standard x Conc of standard
The STD Conc is 200 mg/dl or may vary depending on the reagent brand.
Normal Range

Quick Notice

M. Sulieman

mohammad@mlsgaate.com

standard operating procedures (SOPs). SOPs are detailed instructions that describe how to perform specific laboratory procedures. They ensure that all laboratory staff perform procedures in the same way, which helps to ensure accuracy and reliability.

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