Purpose of the Test
To determine the level of total protein in serum samples.
Principle
Protein in the sample forms a colored complex with cupric ions in an alkaline solution. The intensity of the color formed is proportional to the total protein concentration.
Equipments
1. Semi-autoanalyzer
2. Cuvettes
3. Micropipettes
4. Timer
5. Spectrophotometer
6. Total Protein Reagent (Alkaline copper tartrate )
Sample Type
Serum or plasma is acceptable for the Test
Procedure
1. in 3 clean dry tubes add reagents and sample as mentioned in the Table below
| Blank | STD | TEST | |
|---|---|---|---|
| Total Protein Reagent | 1000 μl | 1000 μl | 1000 μl |
| STD | – | 20 μl | – |
| Sample | – | – | 20 μl |
Procedure
2. Mix Well and incubate for 10 mins at room Tempreature or 5 min @ 37c’
3. using the colorimeter or spectrophotometer read the absorbance against blank at filter wavelenght 540 nm
4. then do the calculations based on the following formula
Total Protein =
Absorbance of test / Absorbance of standard x Conc of standard
The STD Conc is 6 g/dl or may vary depending on the reagent brand.
Normal Range
Quality Control:
1. Run two levels of control with each batch of samples.
2. Results should fall within established confidence limits.
3. Review QC results and take corrective action if needed.
Warning :
1. Contamination can affect results.
2. Hemolysis and lipemia can cause interference.
M. Sulieman
mohammad@mlsgaate.com
Check protein reagent expiration and storage. Inspect instrument calibration. Follow SOP for serum total protein testing.
تحقق من انتهاء صلاحية كاشف البروتين وتخزينه. افحص معايرة الجهاز. اتبع الإجراء المعياري القياسي (SOP) لاختبار البروتين الكلي في الدم.
