Test Catageory
فئة التحليل
1. Clinical Chemisty
Refrence Books
كتب مراجع
1. Bishop’s Clinical chemistry Techniques
Skills Required
المهارات المطلوبة
1. Pipetting
2. Quality Control
Video Resources
مصادر فيديو
Principle
The serum creatinine test relies on Jaffe’s reaction, where creatinine reacts with picric acid in alkaline medium and forms an orange color. The amount of creatinine in the blood is determined by the color intensity using a spectrophotometer. High levels of creatinine may suggest kidney damage or impairment.
Equipments
1. Tube
2. 1000, + 100 Ul Automatic Pipette
3. Spectrophotometer / Colorimeter
4. Creatnine Bi Reagent
Sample Type
Serum r Heparinized Plasma is Acceptable to perform the Test
Procedure
1. Prepare working Reagent by Mixing equal volume from R1+R2
2. in clean dry tubes add 1,000 μL
3. Prepare the Chemistry analyzer * You will need stop watch if you are using colorimeter
4. using Pipette take 100 μL from serum sample and add it to the reagent *Note that there is no incubation period outside
5. once you added the sample record reading 1 after 30 Seconds and record reading 2 after anothe 60 seconds
6. Now comes the calcuations step
| Type | Blank | Test | STD |
|---|---|---|---|
| Working Reagent R1+R2 | 1000 μL | 1000 μL | 1000 μL |
| Sample | – | 100 μL | – |
| Standard | – | – | 1000 μL |
Another 60 Seconds Reading 2
Calculations
Serum Creatnine = Reading 2 of test – Reading 1 of test / Reading 2 of std – Reading 1 of STD x STD Conc
Unit & Normal Range
Unit is : mg/dl
Normal Range: Adults : 0.6 – 1.1 mg/dl
Children: 0.4 – 1.0 mg/dl
M. Sulieman
mohammad@mlsgaate.com
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