Test Catageory
فئة التحليل
Microbiology
Refrence Books
كتب مراجع
1. Bailey & Scott’s Diagnostic Microbiology
2. Laboratory Manual and Workbook in Microbiology
Skills Required
المهارات المطلوبة
1. Inoculation technique
2. Result interpretation
Video Resources
مصادر فيديو
Principle
KIA contains lactose, sucrose, glucose, and iron salts. Utilization of these substrates is detected by associated pH changes of phenol red indicator. Blackening of medium occurs with H2S produced from sulfur-containing amino acids. Gas bubbles are visible.
Solutions & Equipments
1. KIA slants
2. Bacterial isolate
3. Inoculating wire loop
4. Incubator
Sample Type
Pure bacterial isolates from primary culture requiring identification.
Procedure
1. Heavily inoculate slant surface in a zig zag line
2. Stab butt by stabbing straight down to the bottom
3. Loosen any bubbles with needle
4. Incubate slants aerobically at 35±2°C for 18–24 hours
Results
– Yellow slant and butt = acid production from glucose fermentation.
– No change in slant = no lactose/sucrose fermentation.
– Blackening = H2S production. Gas bubbles = gas production.
If yellow butt and RED slant:
Indicates acid production from glucose fermentation in the butt
No acid production from lactose/sucrose in the slant
Lactose/sucrose non-fermenter
If RED butt and yellow slant:
No acid production from glucose in the butt
Acid production from lactose/sucrose fermentation in the slant
Lactose/sucrose fermenter
Notes:
Check reactions using known control organisms
Ensure adequate inoculum and incubation
M. Sulieman
mohammad@mlsgaate.com
Check reactions using known control organisms
Ensure adequate inoculum and incubation
