Test Catageory
فئة التحليل
Routine
Refrence Books
كتب مراجع
1. Monica for Medical Laboratory
Skills Required
المهارات المطلوبة
1. Microscopy
2. Interpretation skills
Video Resources
مصادر فيديو
Principle
Stool analysis involves the visual and microscopic examination of a fecal sample to detect abnormalities and diagnose gastrointestinal issues. Chemical tests may also be done to identify digestive disorders.
Equipments
– Stool collection container
– Applicator sticks
– Microscope slides and coverslips
– Light microscope
– Test tubes and racks
– Chemical reagent test strips (occult blood, pH etc)
– Gloves, face masks, lab coat
Sample Type
Fresh Stool Sample Collected less than 2 Hours
Procedure
1. Collect stool sample in a clean, dry container, avoid contamination
2. Note color, consistency, presence of blood, mucus, parasites
3. Use applicator stick to collect small portion of sample
4.Prepare a thin smear on microscope slide, add drop of saline and coverslip
5. Stain slide if indicated using Gram stain, acid fast stain etc
6. Examine slide under microscope using 10x and 40x objectives
7. Identify pus cells, RBCs, macrophages, parasites, yeasts, bacteria
8. Perform chemical analysis like occult blood, pH as per protocol
9. Compare results to reference ranges
10. Report all findings
Macroscopic Examination:
Parameters:
Colour – brown, yellow, green, red, black, clay colored
Consistency – formed, semi-formed, liquid
Texture – smooth, soft, hard lumps
Odour
Presence of blood, mucus, parasites
Findings:
Pale stool could indicate absence of bile, liver issues, celiac disease
Black tarry stool signifies upper GI bleeding
Foul smelling stool suggests infection or malabsorption
Greasy frothy stool seen in malabsorption
Microscopic Examination:
Parameters:
Pus cells
RBCs
Undigested Food :
Muscle fibers
Vegetables cells
Macrophages
Fat droplets
Parasites like eggs, cysts, trophozoites
Yeast and bacteria
Neutral fat stains
Findings:
Increased pus cells indicate inflammation or infection
Macrocytes and vegetable cells make digestion problems likely
High fat droplets suggest fat malabsorption
Parasites, yeasts, bacteria confirm parasitic infections
Notes:
1. Document all findings in patient chart
2 Include quantitative and qualitative results
3. Ensure timely delivery to requesting physician
M. Sulieman
mohammad@mlsgaate.com
Stool analysis is an important laboratory test that allows visual and microscopic examination of a fecal sample, providing valuable information about the functioning of the gastrointestinal system.