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Stool Analysis

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INTRODUCTION

Stool analysis is an important laboratory test that allows visual and microscopic examination of a fecal sample, providing valuable information about the functioning of the gastrointestinal system. Multiple parameters are analyzed to detect presence of infections, inflammation, bleeding, malabsorption issues or malignancy. The test encompasses both macroscopic evaluation where the color, consistency, odor and presence of blood or mucus are noticed. Microscopic identification involves preparing smears of the stool sample on slides and detecting red blood cells, white blood cells, fat droplets, muscle fibers, parasites and other elements. Chemical testing can also be done using reagents strips for occult blood, pH, enzymes etc. This noninvasive screening helps determine causative agents in diarrhea, constipation and presents a better picture about the intestinal environment. Correlation with the patient’s symptoms and medical history is however needed for a confirmatory diagnosis. Thus, stool examination serves as a simple, cost effective initial investigation to recognize gastrointestinal abnormalities.

Test Catageory
فئة التحليل

Routine

Refrence Books
كتب مراجع

1. Monica for Medical Laboratory

Skills Required
المهارات المطلوبة

1. Microscopy
2. Interpretation skills

Video Resources
مصادر فيديو

Resource 1
عربي

Principle

Stool analysis involves the visual and microscopic examination of a fecal sample to detect abnormalities and diagnose gastrointestinal issues. Chemical tests may also be done to identify digestive disorders.

Equipments

– Stool collection container
– Applicator sticks
– Microscope slides and coverslips
– Light microscope
– Test tubes and racks
– Chemical reagent test strips (occult blood, pH etc)
– Gloves, face masks, lab coat

Sample Type

Fresh Stool Sample Collected less than 2 Hours

Procedure

1. Collect stool sample in a clean, dry container, avoid contamination
2. Note color, consistency, presence of blood, mucus, parasites
3. Use applicator stick to collect small portion of sample
4.Prepare a thin smear on microscope slide, add drop of saline and coverslip
5. Stain slide if indicated using Gram stain, acid fast stain etc
6. Examine slide under microscope using 10x and 40x objectives
7. Identify pus cells, RBCs, macrophages, parasites, yeasts, bacteria
8. Perform chemical analysis like occult blood, pH as per protocol
9. Compare results to reference ranges
10. Report all findings

Macroscopic Examination:

Parameters:
Colour – brown, yellow, green, red, black, clay colored
Consistency – formed, semi-formed, liquid
Texture – smooth, soft, hard lumps
Odour
Presence of blood, mucus, parasites


Findings:
Pale stool could indicate absence of bile, liver issues, celiac disease
Black tarry stool signifies upper GI bleeding
Foul smelling stool suggests infection or malabsorption
Greasy frothy stool seen in malabsorption

Microscopic Examination:
Parameters:

Pus cells

RBCs

Undigested Food :
Muscle fibers
Vegetables cells

Macrophages
Fat droplets
Parasites like eggs, cysts, trophozoites

Yeast and bacteria
Neutral fat stains
Findings:
Increased pus cells indicate inflammation or infection
Macrocytes and vegetable cells make digestion problems likely
High fat droplets suggest fat malabsorption
Parasites, yeasts, bacteria confirm parasitic infections

Notes: 

1. Document all findings in patient chart
2 Include quantitative and qualitative results
3. Ensure timely delivery to requesting physician

Quick Notice

M. Sulieman

mohammad@mlsgaate.com

Stool analysis is an important laboratory test that allows visual and microscopic examination of a fecal sample, providing valuable information about the functioning of the gastrointestinal system.

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