TWBCs Correction

1 min read

INTRODUCTION

White blood cells, also known as leukocytes, are an important part of the immune system and help fight infection. A total white blood cell (WBC) count is a common medical test that gives the number of white blood cells in a sample of blood. The normal range for a total WBC count is between 4,500 and 11,000 cells per microliter.
in some cases of anemia the TWBCs is falsely increased, because of the released Nucleated red blood cells . so we need to correct the obtained TWBCs Count before reporting the result.

Test Catageory
فئة التحليل

Hematology

Refrence Books
كتب مراجع

1. Daice & Lewis Practical Hematology

Skills Required
المهارات المطلوبة

1. Microscopy
2. Calculation

Video Resources
مصادر فيديو

Resource 1

Principle

The total WBC count can be inaccurate if there are nucleated RBCs (NRBCs) or other immature cell types present in the blood. A correction factor must be applied to get an accurate TWBC.

Equipments

1. Microscope
2. Oil
3. Thin Blood Film Stained with (Wright Stain like Giems or Lieshman Stain)
4. Counter
5. Pen & Paper

Sample Type

Whole Blood (EDTA)

Procedure

1.      Put oil in the thin blood film and use x 100 oil immersion for counting (you can use x40 for correction but you need to put coverslip over the oil to protect the lens from oil.
2.      In the ideal area of slide Count 100 white blood cells including the Nucleated RBCs
3.      After finishing 100 cells calculate the number of WBCs and the Number of NRBCs
4.      Use the following calculation formula to obtain the Corrected TWBCs Count